
@inbook {82598,
	title = {Patterned Optogenetic Stimulation Using a DMD Projector.},
	booktitle = {Channelrhodopsin},
	volume = {2191},
	number = {Methods Mol Biol},
	year = {2021},
	month = {2021},
	pages = {173-188},
	publisher = {Humana-Springer},
	organization = {Humana-Springer},
	abstract = {<p>Optical manipulation is a powerful way to control neural activity in vitro and in vivo with millisecond precision. Patterning of light provides the remarkable ability to simultaneously target spatially segregated neurons from a population. Commercially available projectors provide one of the simplest and most economical ways of achieving spatial light modulation at millisecond timescales. Here, we describe the protocol for constructing a projector-based spatio-temporal light patterning system integrated with a microscope on a typical electrophysiology rig. The set-up is well suited for applications requiring rapid, distinct, and combinatorial inputs, akin to brain activity. This equipment involved is fairly economical (\&lt;$5000 including all optical and mechanical components), and the set-up is easy to assemble and program.</p>
},
	isbn = {978-1-0716-0829-6},
	doi = {10.1007/978-1-0716-0830-2_11},
	author = {Bhatia, Aanchal and Moza, Sahil and Bhalla, Upinder S}
}
