
@article {62768,
	title = {Measurement of Store-Operated Calcium Entry in Human Neural Cells: From Precursors to Differentiated Neurons.},
	journal = {Methods Mol Biol},
	volume = {2029},
	year = {2019},
	month = {2019},
	pages = {257-271},
	abstract = {<p>Calcium imaging in an ex-vivo\&nbsp;setup is used to understand the calcium status of isolated cells or tissue. In this chapter we explain the use of the ratiometric chemical indicator Fura-2 which can be loaded into isolated cells in the form of lipophilic acetomethyl (AM) esters. Fura-2 is a combination of calcium chelator and fluorophore, and can be used with dual wavelength excitation (340/380\&nbsp;nm) for quantitative calcium concentrations. The cells can then be viewed using a fluorescence microscope and captured by a CCD camera. We specifically discuss the technique involved in understanding the endoplasmic reticulum (ER)-driven store-operated calcium entry (SOCE) in human neural precursors (NPCs)\&nbsp;and spontaneously differentiated neurons derived from a pluripotent human\&nbsp;embryonic stem cell\&nbsp;(hESC) line. The derivation of neural precursors from stem cells and their subsequent spontaneous neural differentiation is also explained. The method can be used for various non-excitable and excitable cell types including neurons, be it freshly isolated, from frozen vials, or derived from different stem cell lines.</p>
},
	issn = {1940-6029},
	doi = {10.1007/978-1-4939-9631-5_20},
	author = {Gopurappilly, Renjitha and Deb, Bipan Kumar and Chakraborty, Pragnya and Hasan, Gaiti}
}
