
@article {24299,
	title = {A structural map of oncomiR-1 at single-nucleotide resolution.},
	journal = {Nucleic Acids Res},
	volume = {45},
	year = {2017},
	month = {2017 Sep 19},
	pages = {9694-9705},
	abstract = {<p>The miR-17-92a cluster, also known as {\textquoteright}oncomiR-1{\textquoteright}, is an RNA transcript that plays a pivotal regulatory role in cellular processes, including the cell cycle, proliferation and apoptosis. Its dysregulation underlies the development of several cancers. Oncomir-1 comprises six constituent miRNAs, each processed with different efficiencies as a function of both developmental time and tissue type. The structural mechanisms that regulate such differential processing are unknown, and this has impeded our understanding of the dysregulation of oncomiR-1 in pathophysiology. By probing the sensitivity of each nucleotide in oncomiR-1 to reactive small molecules, we present a secondary structural map of this RNA at single-nucleotide resolution. The secondary structure and solvent accessible regions of oncomiR-1 reveal that most of its primary microRNA domains are suboptimal substrates for Drosha-DGCR8, and therefore resistant to microprocessing. The structure indicates that the binding of trans-acting factors is required to remodel the tertiary organization and unmask cryptic primary microRNA domains to facilitate their processing into pre-microRNAs.</p>
},
	issn = {1362-4962},
	doi = {10.1093/nar/gkx613},
	author = {Chakraborty, Saikat and Krishnan, Yamuna}
}
