
@article {152168,
	title = {Motor Function of the Two-Component EEA1-Rab5 Revealed by dcFCCS.},
	journal = {Methods Mol Biol},
	volume = {2881},
	year = {2025},
	month = {2025},
	pages = {87-115},
	abstract = {<p>Fluorescence correlation spectroscopy (FCS) enables the measurement of fluctuations at fast timescales (typically few nanoseconds) and with high spatial resolution (tens of nanometers). This single-molecule measurement has been used to characterize single-molecule transport and flexibility of polymers and biomolecules such as DNA and RNA. Here, we apply this technique as dual-color fluorescence cross-correlation spectroscopy (dcFCCS) to identify the motor function of the tethering protein EEA1 and the small GTPase Rab5 by probing the flexibility changes through end-monomer fluctuations.</p>
},
	keywords = {Humans, rab5 GTP-Binding Proteins, Single Molecule Imaging, Spectrometry, Fluorescence, Vesicular Transport Proteins},
	issn = {1940-6029},
	doi = {10.1007/978-1-0716-4280-1_4},
	author = {Soler, Joan Antoni and Singh, Anupam and Zerial, Marino and Thutupalli, Shashi}
}
