
@article {105898,
	title = {Two photon imaging of calcium responses in murine Purkinje neurons.},
	journal = {STAR Protoc},
	volume = {3},
	year = {2022},
	month = {2022 Mar 18},
	pages = {101105},
	abstract = {<p>Purkinje neurons (PNs) are an important component of the motor learning and coordination circuit and are affected in spino-cerebellar ataxias. Maintaining healthy PNs in cerebellar slices and recording their Ca transients can be challenging. Here, we describe a protocol for measuring Ca transients in PNs from adult mice, including problem-solving tips. This protocol can be used to measure neuronal excitability and agonist-mediated Ca signaling in cerebellar slices expressing a genetic Ca reporter in all PNs, thus improving yield of data. For complete details on the use and execution of this profile, please refer to Dhanya and Hasan (2021).</p>
},
	issn = {2666-1667},
	doi = {10.1016/j.xpro.2021.101105},
	author = {Dhanya, Sreeja Kumari and Hasan, Gaiti}
}
