
@article {1029,
	title = {A tale of two paralogs: human Transformer2 proteins with differential RNA-binding affinities.},
	journal = {J Biomol Struct Dyn},
	volume = {34},
	year = {2016},
	month = {2015 Nov 26},
	pages = {1979-86},
	chapter = {1979},
	abstract = {<p>The Transformer2 (Tra2) proteins in humans are homologues of the Drosophila Tra2 protein. One of the two RNA-binding paralogs, Tra2β, has been very well-studied over the past decade, but not much is known about Tra2α. It was very recently shown that the two proteins demonstrate the phenomenon of paralog compensation. Here, we provide a structural basis for this genetic backup circuit, using molecular modelling and dynamics studies. We show that the two proteins display similar binding specificities, but differential affinities to a short GAA-rich RNA stretch. Starting from the 6-nucleotide RNA in the solution structure, close to 4000 virtual mutations were modelled on RNA and the domain-RNA interactions were studied after energy minimisation to convergence. Separately, another known 13-nucleotide stretch was docked and the domain-RNA interactions were observed through a 100-ns dynamics trajectory. We have also demonstrated the {\textquoteright}compensatory{\textquoteright} mechanism at the level of domains in one of the domain repeat-containing RNA-binding proteins.</p>
},
	issn = {1538-0254},
	doi = {10.1080/07391102.2015.1100551},
	author = {Ghosh, Pritha and Grellscheid, Sushma Nagaraja and Sowdhamini, R}
}
