
@article {101901,
	title = {In Vitro Anticancer Activity of  Root{\textquoteright}s Extract toward Human Cervical Cancer and Identification of Potential Bioactive Compounds.},
	journal = {Biomed Res Int},
	volume = {2021},
	year = {2021},
	month = {2021},
	pages = {4259777},
	abstract = {<p>is traditionally used to cure several diseases including cancer, wounds, and hypertension. The present study was designed to investigate the anticancer activity of the methanolic root extract of (IC-MeOH). The water-soluble tetrazolium-1 and colony formation assays were used to check the proliferation ability of the cells. Cell apoptosis and cell cycle were measured by flow cytometry-based fluorescence-activated cell sorting. The ultrahigh-performance liquid chromatography-high-resolution mass spectrometry (UHPLC-HRMS) analysis was used for the metabolites profiling of IC-MeOH. Based on high-mass accuracy, spectral data, and previous reports, tentative compound identifications were assigned. Our findings revealed that IC-MeOH inhibited the proliferation of HeLa and CaSki cells. The plant extract was also found to induce a concentration- and time-dependent apoptosis and cell cycle arrest in the G0/G1 phase (IC value) in CaSki cell line. Analysis of IC-MeOH permitted the identification of 10 compounds already reported for their anticancer activity, epicatechin, curcumin, (-)-yatein, caffeic acid, myricetin, jatrorrhizine, harmaline, cinnamaldehyde, dobutamine, and syringin. In conclusion, IC-MeOH is a rich source of cytotoxic metabolites that inhibits human cervical cancer proliferation apoptosis and cell cycle arrest.</p>
},
	issn = {2314-6141},
	doi = {10.1155/2021/4259777},
	author = {Nayim, Paul and Sudhir, Krishna and Mbaveng, Armelle T and Kuete, Victor and Sanjukta, Mukherjee}
}
