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Central Imaging and Flow Cytometry Facility
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Biological systems resembles plethora of nanoscale structures to carry out demanding tasks of localized signal reception and transduction, membrane transport, gene expression and computation in the context of the cellular environment. From our understanding of cellular processes it appears that construction of these nano-architectures in active cellular environments are critical for all cell function. At NCBS we have an interactive research program that combines diverse groups interested in nanonmeter scale of organization in living cells. This program includes researches from diverse backgrounds ranging from cell and neuro-bilogy, protein structure and soft-condensed matter physics. |
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NCBS is structured along the lines of understanding different scales of organization in biology from systems level neuroscience to the single molecule scale. This demands continuous augmentation of the infrastructure capabilities in terms of specific research equipment necessary for the study of the nano-scale in biology. It serves to exploit a common theme in the work of different researchers at NCBS. This resolves around the development of methodologies o observe nano-scale architecture and phenomena in living systems in real time to develop and deploy the methodologies required for visualization of sub-cellular and cellular processes at temporal and spatial resolution previously unachieved. Similarly, it is increasingly becoming imperative to analyze and sort/purify the cell/organelle populations of interest by high-speed flow cytometry to study the genomic, proteomic and functional aspects of such purified populations. |
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In India, there are limited opportunities to promote organized training and knowledge dissemination in the filed of imaging. We do not believe in operator assisted imaging or flow cytometry facilities as it would not be practical for any operator/s to understand and develop interest in all of the research projects of a institution and run those experiments in a given time frame. Therefore, we believe in training the individual researchers to operate microscopes or flow cytometers available in NCBS. To achieve this goal, we have started a state-of–the-art imagining and flow cytometry facility that will not only cater our institutional needs but also provide training to researchers coming from all over India and. abroad. |
Imaging Stations available at NCBS
LSM 5 LIVE Duo Scan Ccontocal Microscope- Carl ZEISS
LSM 510 meta confocal microscope with multiphoton laser-Carl ZEISS
LSM 510 meta confocal microscope with 405 laser-Carl ZEISS
FV1000 Confocal Microscope with Simultaneous Scanner-Olympus
Yokagawa Spinning Disc Confocal Microscope-Andor & Olympus
FV1000 Confocal Microscope-Olympus
Zoom Stereo Microscope SZX16-Olympus
Macro Zoom Microscope MVX10-Olympus
Atomic Force Microscope-Molecular Imaging
Near Field Microscope-Witech
Microinjection setup-Nikon & Eppendorf
Transmission Electron Microsope - FEI
Flow Cytometers available at NCBS
FACS Calibur- Becton Dickinson
FACS Vantage SE-Becton Dickinson
FACS Aria-Becton Dickinson
CyAn ADP-Becman Coulter
Guava Easy Cyte - Merck-Millipore
BD-NCBS centre of excellence in flow cytometry:
This centre runs flow cytometry training programs for students coming from all over India. For details contact Dr. H. Krishnamurthy (krishna at ncbs dot res dot in) or Dr. Paresh Jain (paresh_jain@bd.com) or Mr. Ubaldo Barbosa (ubaldo_barbosa@bd.com).
How do I sign up?
NCBS users can signup at http://conflow.ncbs.res.in. Non-NCBS users can contact H. Krishnamruthy by e-mail krishna at ncbs dot res dot in or call 2366 6013 or 6717 6013. For assissted analysis call the facility at least 24 hours before you schedule an appoinment. Users cannot cancel an appointment less than 24 hours in advance. All appoinments are made on a first-come-first-served basis, with NCBS students given scheduling priority.
Contact Information
Facility Incharge:
H. Krishnamurthy
National Center for Biological Sciences
UAS-GKVK Campus, New Bellary Road
Bangalore 560 065
Tel: 91-80-2366-6013 or 91-80-6717-6013
Fax: 91-80-2363-6662
e-mail: krishna at ncbs dot res dot in
Research and Development
Manoj Mathew
Facility Crew
Navya Jain
Deepthy G. Rao
Harikrishna A
Bhavyashree Suresh
Pavana G Rotti
Anil Kumar HV
Advisory Committee
Apurva Sarin
Yamuna Krishnan
Satyajit Mayor
Acknowledgement of the Facility
The advisory committee would recommend the users to acknowledge the facility in their scientific publications.